Wilburworld of Science

Activity:  Finding a Gene on the Chromosome Map - Students use a pedigree and jigsaw puzzle to explore how scientists use genetic information from genomic scans and a family to identify a gene associated with a genetic disorder. 

After completing this activity, students will
  • know different modes of inheritance 
  • understand how pedigrees can be used to identify a gene associated with a genetic disorder and map it to a specific chromosome location
Reading/Class Discussion:  ​ Genetic Testing for Huntington's Disease - Part 1 - A Family Disease - Students learn about genetic mutation that causes this disorder, how it causes a dysfunctional protein to be made, the effect that it has on particular nerve cells in the brain, and ultimately how it results in the classic symptoms of this disease.  

After completing these activities, students will 
  • know how a genetic mutation results in the symptoms associated with Huntington's disease

Activity:  ​ Genetic Testing for Huntington's Disease - Part 2 - Jenny's Chances - Jenny's Chances Students use statistics/punnet squares and knowledge of pedigrees and inheritance patterns to assess Jenny's chances of having this disease.  

After completing these activities, students will 
  • be able to determine Jenny's risk or chance of developing this disease
Activity:  ​ Genetic Testing for Huntington's Disease - preparation for part 5 - Students learn how to use a micropipette for accurate measurements and transference of DNA samples at the microliter scale

After completing this activity, students will
  • be able to use a micropipette to accurately measure and transfer small amounts of liquid
Activity: ​ Genetic Testing for Huntington's Disease - Part 3 - Jenny's Family - Jenny's Family History - Students continue risk assessment using a pedigree chart.  
Activity/Class DIscussion: ​ Genetic Testing for Huntington's Disease - Part 4 - Jenny's Choices -  Ethical and personal discussion of the pro's and con's to this type of decision for adults and children. 
Activity:  ​ Genetic Testing for Huntington's Disease - preparation for part 5 - Students learn how to prepare agarose gel for electrophoresis.

After completing this activity, students will
  • be able to create an agarose gel mold for electrophoresi
PPT / Notes/Lecture: continued - Genetic Testing, DNA basics.  A brief presentation aimed at the topics necessary for students to understand the fundamental techniques and strategies used to test for genetic disorders or predispositions.  
Animation:        Huntington Protein Dysfunction: - Simulation of Huntington protein malfunction in a brain cell.  
Lab/Class Discussion: ​ Genetic Testing for Huntington's Disease - Part 5 - The Genetic Test - Students load and run DNA samples using agarose gel electrophoresis as a paper simulation.  This technique is used to separate and analyze the size of DNA molecules.  Students determine which family members are carrying the Huntington allele.  

After completing this activity, students will
  • understand how PCR makes multiple copies of a specific gene
  • understand how electrophoresis separates molecules according to their size
Activity/Class Discussion: ​ Genetic Testing for Huntington's Disease - Part 5 - The Genetic Test -  Students run a gel, then assess test results to determine which family members are carrying the Huntington allele.  

After participating in these activities, students will
  • know agarose gel elecrophoresis, PCR, STR, CNV
  • understand that much of our DNA contains repeat sequences and how variable amounts of these sequences can result in protein malfunction
  • understand how specific DNA sequences are located, copied & visualized for analysis
Activity:  Function Finders, Protein Profiles -   Students transcribe and translate genes to determine the resulting amino acid sequence and the 3-dimensional proteins it folds to form.  Students relate each protein to its function in the cell. 

After completing this activity, student will
  • understand that even a single bp mutation in DNA can alter a protein's shape/function which can result in a disorder
  • understand the role of enzymes in reactions

Watch:  HOUSE Episode -


B         

Disease Diagnostics         
Unit 3: Genetic Diseases/Disorder
Single Gene Mutations
Feb 24, Mon, Day E
Feb 25, Tues, Day F
Feb 26, Wed, Day A
Feb 27, Thur, Day B
Feb 28, Fri, Day C
HW
Mar 3, Mon, Day D
Mar 4, Tues, Day E
Review Worksheet - Pedigrees & Inheritance Patterns - A brief review of autosomal dominant, autosomal recessive, and X-linked traits. 
HW
Case Study Analysis - Pedigrees & Inheritance Patterns 
Activity - DNA & RNA review - Students use paper DNA & RNA nucleotides to model 
  • the structure of a double helix
  • the process of DNA replication 
  • the process of transcription, and explain its role in protein synthesis
Alternate Assignment:  
Mar 5, Wed, Day F
PPT / Notes/Lecture: continued - Genetic Testing, DNA basics.  A brief presentation aimed at the topics necessary for students to understand the fundamental techniques and strategies used to test for genetic disorders or predispositions.  

After participating in these activities, students will
  • know agarose gel elecrophoresis, PCR, STR, CNV
  • understand that much of our DNA contains repeat sequences and how variable amounts of these sequences can result in protein malfunction
  • understand how specific DNA sequences are located, copied & visualized for analysis
Mar 7, Fri, Day B
Mar 10, Mon, Day C
Mar 11, Tues, Day D